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Spleen tyrosine Kinase (SYK) (pTyr323) antibody

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Antigen
  • p72-Syk
  • Sykb
  • p72syk
Alternatives
anti-Human Spleen tyrosine Kinase antibody for Immunocytochemistry
Epitope
pTyr323
48
33
30
22
21
17
16
15
15
13
11
10
10
9
8
7
7
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
345
133
110
9
7
5
5
5
2
1
1
Host
Rabbit
253
86
19
Clonality
Polyclonal
Conjugate
Un-conjugated
13
12
11
8
8
6
3
3
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC)
266
134
117
58
56
44
24
22
15
7
4
4
3
2
2
2
1
1
1
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Immunogen Peptide sequence around phosphorylation site of pTyr323 (N-P-Y (p) -E- P) derived from Human syk. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of syk only when phosphorylated at tyrosine 323.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name syk (SYK Antibody Abstract)
Background Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Molecular Weight 72 kDa
NCBI Accession NP_001128
UniProt P43405
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Zhang, Billingsley, Kincaid et al.: "Phosphorylation of Syk activation loop tyrosines is essential for Syk function. An in vivo study using a specific anti-Syk activation loop phosphotyrosine antibody." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35442-7, 2000 (PubMed).

Turner, Schweighoffer, Colucci et al.: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

Deckert, Elly, Altman et al.: "Coordinated regulation of the tyrosine phosphorylation of Cbl by Fyn and Syk tyrosine kinases." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8867-74, 1998 (PubMed).

Law, Chandran, Sidorenko et al.: "Phospholipase C-gamma1 interacts with conserved phosphotyrosyl residues in the linker region of Syk and is a substrate for Syk." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1305-15, 1996 (PubMed).