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Antibodies
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anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody
| Antigen |
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| Synonyms |
NS5, CRAF, Raf-1, c-Raf, craf, c-raf, RAF1, MGC165882, raf1, DKFZp469F0314 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. |
ABIN362493 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
250.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Raf1 (Ab-259)
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Swiss-Prot
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P04049
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Immunogen
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The antiserum was produced against synthesized non-phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-S P -T-P).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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Raf1 (Ab-259) (Human) antibody detects endogenous levels of total Raf-1 protein.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1)", type "Antibodies"
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Hosts
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Rabbit (119), Mouse (14), Rat (5), Chicken (1)
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Reactivities
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Human (136), Mouse (Murine) (43), Rat (Rattus) (32), Cow (Bovine) (5), Chicken (3), Dog (Canine) (3), Monkey (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (104), Enzyme Immunoassay (EIA) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (43), Immunohistochemistry (IHC) (28), Dot Blot (Dot) (19), ELISA (18), Immunofluorescence (IF) (4), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), ELISA (Detection) (1)
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Epitopes
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pSer338 (12), N-Term (6), pSer259 (6), pSer642 (3), S296 (2), S43 (2), S471 (2), T269 (2), Y340 (2), pSer296 (2), pSer301 (2), pSer339 (2), pSer43 (2), pSer471 (2), pSer494 (2), pThr269 (2), pTyr340 (2), pTyr341 (2), S233 (1), S259 (1), S338 (1), S494 (1), S621 (1), Y341 (1), pSer233 (1), pSer239 (1), pSer621 (1)
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