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MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 392-396)

Details for Product anti-MAP2K2 Antibody No. ABIN362497, Supplier: Log in to see
Antigen
Alternatives
anti-Human MAP2K2 antibody for Western Blotting
Epitope
AA 392-396
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2
2
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2
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
232
108
91
8
4
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Host
Rabbit
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38
9
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
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Application
Immunohistochemistry (IHC), Western Blotting (WB)
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39
27
26
24
15
13
11
7
4
2
1
1
Supplier
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Immunogen Peptide sequence around AA 392-396 (P-G-T-P-T) derived from Human MEK-2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total MEK2 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name MEK-2 (MAP2K2 Antibody Abstract)
Background Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases
Molecular Weight 44 kDa
NCBI Accession NP_109587
UniProt P36507
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Rosen, Ginty, Weber et al.: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." in: Neuron, Vol. 12, Issue 6, pp. 1207-21, 1994 (PubMed).

Cowley, Paterson, Kemp et al.: "Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells." in: Cell, Vol. 77, Issue 6, pp. 841-52, 1994 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).