FAK antibody (AA 574-578, AA 575-579)

Catalog No. ABIN363117

Cited in 1 publication. The Rabbit Polyclonal anti-FAK antibody (ABIN363117) specifically detects FAK in IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FAK antibody (AA 574-578, AA 575-579) (ABIN363117)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-FAK Antibody

Binding Specificity: AA 574-578, AA 575-579

Specificity: The antibody detects endogenous level of total FAK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen .

Immunogen: Peptide sequence around AA 574-578, 575-579 (T-Y-Y-K-A) derived from Human FAK. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-FAK antibody (ABIN363117)

Ruan, Liu, Zhang, Yan, Fan, Shen, Wang, Zheng, Wang, Lu: "Luteolin reduces the invasive potential of malignant melanoma cells by targeting ?3 integrin and the epithelial-mesenchymal transition." in: Acta pharmacologica Sinica, Vol. 33, Issue 10, pp. 1325-31, (2012) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: FAK

Background: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Molecular Weight: 125 kDa

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling