SIRT6 antibody (C-Term)

Catalog No. ABIN363528

Product Details anti-SIRT6 Antibody

Target: SIRT6 (Sirtuin 6 (SIRT6))

Binding Specificity: AA 300-355, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT6 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Reacts with human SIRT6 protein

Cross-Reactivity (Details): 72 % sequence identity with rat.

Purification: Antigen affinity purified

Immunogen: Synthetic peptide made to a C-terminal region of the human SIRT6 protein (within residues 300-355)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 2 μg/mL - ICC/IF: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.05 mg/mL

Buffer: Tris-citrate, phosphate, ( pH 7-8) buffer, Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for SIRT6

Target: SIRT6 (Sirtuin 6 (SIRT6))

Abstract: SIRT6 Products

Synonyms: 2810449N18Rik antibody, AI043036 antibody, Sir2l6 antibody, SIR2L6 antibody, zgc:86729 antibody, sirtuin 6 antibody, sirtuin 6 S homeolog antibody, Sirt6 antibody, SIRT6 antibody, sirt6.S antibody, sirt6 antibody

Background: SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.