IL1R1 antibody (Extracellular Domain)

Catalog No. ABIN364253

The Mouse Monoclonal anti-IL1R1 antibody is suitable to detect IL1R1 in samples from Human. It has been validated for IHC, ELISA, IHC (p) and RIA.

Quick Overview for IL1R1 antibody (Extracellular Domain) (ABIN364253)

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Clone: 40101

Product Details anti-IL1R1 Antibody

Binding Specificity: Extracellular Domain

Specificity: These antibodies react with cell surface and soluble human IL-1 receptor, Specific epitopes are unknown, but they do partially inhibit (30-50 % ) binding of one another to IL-1 receptor. They do not block binding of IL-1 to receptor, and they do not cross-react with receptors for human IL-2 or IL-4.

Purification: Protein G purified

Immunogen: The extracellular domain of the type I human IL-1 receptor.

Type of Immunogen: Protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), RIA

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA: Can be used as capture reagents or second antibody at a concentration of 5 μg/mL. RIA: Concentrations of 20-30 ng/mL of these antibodies will bind radiolabeled IL-1 receptor in a radioimmunoassay format. Immunocytochemistry: IL-1 receptor can be detected on cultured cells using antibody concentrations of 10-100 μg/mL.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. Sourced in Ascites.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C,-80 °C

Storage Comment: Short term: -20°C
Long term: -70°C
Avoid freeze-thaw cycles.

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: IL1RA / IL1R1

Background: Name/Gene ID: IL1R1
Family: Interleukin

Synonyms: IL1R1, Antigen CD121a, D2S1473, IL-1R-1, IL-1R-alpha, IL-1RT-1, IL-1RT1, IL1R, IL1RA, IL1RT1, Interleukin receptor 1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, CD121A, CD121a antigen, Interleukin 1 receptor, type I, Interleukin-1 receptor type I, Interleukin-1 type i receptor, p80

Gene ID: 3554

UniProt: P14778

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints