GPX3 antibody (AA 102-114)

Catalog No. ABIN364427

Product Details anti-GPX3 Antibody

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Binding Specificity: AA 102-114

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow, Monkey, Bat, Horse

Host: Goat

Clonality: Polyclonal

Conjugate: This GPX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GPX3. This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%) Rabbit, Opossum, Chicken (92%) Hamster, Zebrafish, Pseudomonas (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NQFGKQEPGENSE from an internal region of human GPX3 (NP_002075.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%), Rabbit, Opossum, Chicken (92%), Hamster, Zebrafish, Pseudomonas (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:1000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GPX3

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Alternative Name: GPX3 (GPX3 Products)

Synonyms: GPX3 antibody, DKFZp469D1932 antibody, AA960521 antibody, EGPx antibody, GPx antibody, GSHPx-3 antibody, GSHPx-P antibody, GPx-3 antibody, GPx-P antibody, Gpxp antibody, glutathione peroxidase 3 antibody, gpx3 antibody, GPX3 antibody, Gpx3 antibody

Background: Name/Gene ID: GPX3

Synonyms: GPX3, Glutathione peroxidase 3, GSHPx-3, GPXP, GSHPx-P, GPx-3, GPx-P, Plasma glutathione peroxidase

Gene ID: 2878

NCBI Accession: NP_002075

UniProt: P22352

Pathways: Thyroid Hormone Synthesis