CD72 antibody (CD72 Molecule)

Details for Product anti-CD72 Antibody No. ABIN371001, Supplier: Log in to see
Antigen
  • CD72c
  • Ly-19
  • Ly-32
  • Ly-m19
  • Lyb-2
  • CD72b
  • LYB2
  • CD72 antigen
  • CD72 molecule
  • Cd72
  • CD72
Reactivity
Mouse (Murine)
129
31
11
Host
Mouse
98
59
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD72 antibody is un-conjugated
18
16
12
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
77
76
46
34
25
22
21
3
3
2
1
1
Options
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Immunogen DBA/2 mouse spleen cells. (Ref. 1)
Clone 10-1-D-2
Isotype IgG2b
Specificity This antibody recognises CD72.1/Lyb-2.1 (Mr. 45 kDa). It blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing. (Ref.1) However, 10-1.D.2 can itself trigger a positive signal by binding CD72. (Ref.1-5)
Characteristics Synonyms: Lyb-2, Ly-32, Ly32, B-Cell marker, B-cell differentiation antigen CD72, Lymphocyte antigen32
Purification Purified
Alternative Name CD72 (CD72 Antibody Abstract)
Background Murine CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells. (Ref.1,2)Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32
Gene ID 100504743
UniProt P21855
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry. (Ref.1,2)Functional Assays: In vitro blocking and proliferation assays. (Ref.2,3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Robinson, Landolfi, Schäfer, Parnes: "Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 9, pp. 4764-72, 1993 (PubMed).

Luo, Van de Velde, von Hoegen, Parnes, Thielemans: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, 1992 (PubMed).

Beavil, Edmeades, Gould, Sutton: "Alpha-helical coiled-coil stalks in the low-affinity receptor for IgE (Fc epsilon RII/CD23) and related C-type lectins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 753-7, 1992 (PubMed).

Snow, Mond, Subbarao: "Enhancement by monoclonal anti-Lyb-2 antibody of antigen-specific B lymphocyte expansion stimulated by TNP-Ficoll and T lymphocyte-derived factors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 6, pp. 1793-6, 1986 (PubMed).

Subbarao, Mosier: "Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 5, pp. 2033-7, 1983 (PubMed).