Flow Cytometry: Identification and enumeration of CD103 + cells. Immunoprecipitation: This antibody exhibits antigen immunoprecipitation patterns similarif not identical to the Rat anti-Mouse CD103 mAb M290. Immunohistochemistry on Frozen Sections. In vitro Activation. See Ref.1 for more details. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS without preservatives.
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
CD103 (ITGAE)
(Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20 % of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. (Ref.1-3) In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150 kDa alpha chain and a 120 kDa beta chain. Integrin alpha E is expressed by approximately 1 % of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.Synonyms: HML-1 antigen, ITGAE, Integrin alpha-E, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen