Bcl-2 antibody (AA 61-76)

Catalog No. ABIN371613

This anti-Bcl-2 antibody is a Mouse Monoclonal antibody detecting Bcl-2 in WB and EIA. Suitable for Mouse and Rat.

Quick Overview for Bcl-2 antibody (AA 61-76) (ABIN371613)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 10C2

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 61-76

Specificity: This antibody reacts with Bcl-2 (Mr. 26 kDa). The monoclonal antibody 10C2 reacts with an epitope between amino acids 61-76 of Mouse Bcl-2. The antibody also reacts with Rat Bcl-2 (Ref.1,14).

Characteristics: Synonyms: BCL2, Bcl-2 alpha, Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2

Purification: Purified

Immunogen: KLH-conjugated peptide corresponding to 61-76 amino acid sequence of murine Bcl-2. (Ref. 1)

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot: < / = 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 100 mM Borate buffered saline, pH 8.2 without preservatives or amine-containing buffer salts.

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background: Bcl-2 is a 26 kDa member of the family of proteins involved in regulation of programmed cell death, or apoptosis. It is expressed in a variety of both normal and neoplastic tissues and appears to be membranebound since it is detectable in crude membrane and nuclear fractions, but not in the soluble fraction of cell lysates. Overexpression of Bcl-2 can prevent apoptosis, while formation of heterodimers with Bax, another Bcl-2-related protein, inhibits Bcl-2's ability to promote cell survival. Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (Ref.2) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (Ref.3) Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, gamma-irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha

UniProt: P10417

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling