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KRT18 antibody (Keratin 18) (N-Term)

Details for Product anti-KRT18 Antibody No. ABIN371615, Supplier: Log in to see
Antigen
  • cb83
  • CK18
  • CYK18
  • dapk1
  • DreK18
  • K18
  • Krt1-18
  • krt18
  • KRT18
  • krtt1c6
  • MGC64569
  • MGC75922
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • zgc:66015
Alternatives
anti-Mammalian KRT18 antibody for Immunocytochemistry
Epitope
N-Term
55
30
25
22
21
14
13
9
9
8
8
8
6
5
5
5
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mammalian
899
166
143
57
55
31
29
25
20
19
19
18
13
12
7
7
7
5
4
3
3
3
1
1
1
Host
Mouse
664
268
10
9
6
2
Clonality (Clone)
Monoclonal ()
Conjugate
This KRT18 antibody is un-conjugated
50
47
21
18
18
17
14
14
14
14
14
14
14
14
14
14
11
11
4
3
3
3
3
3
3
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
625
447
377
339
311
272
251
193
164
34
18
11
11
9
3
2
2
1
1
1
Supplier
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Immunogen Recombinant N-terminal fragment of keratin 18.
Clone SB38b
Isotype IgG2b
Specificity This antibody reacts specifically specifically with Keratin 18 and recombinant N-terminal Keratin 18.
Characteristics Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18, Cell proliferation-inducinggene 46 protein, Keratin type I cytoskeletal 18
Purification Purified
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30 % sequence homology. (Ref.1-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein of approximately 48 kDa. It exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia, and is often co-expressed with cytokeratin 8. (Ref.6-8) Tissues from the gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
UniProt P05783
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA: 1/5,000-1/20,000. Western Blot: 1/2,000-1/4,000. Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections): 10 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.0 without preservatives or amine-containing buffer salts.
Preservative Without preservative
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Ku, Darling, Krams, Esquivel, Keeffe, Sibley, Lee, Wright, Omary: "Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 10, pp. 6063-8, 2003 (PubMed).

Kulesh, Oshima: "Complete structure of the gene for human keratin 18." in: Genomics, Vol. 4, Issue 3, pp. 339-47, 1989 (PubMed).

Steinert, Roop: "Molecular and cellular biology of intermediate filaments." in: Annual review of biochemistry, Vol. 57, pp. 593-625, 1988 (PubMed).

Schiller, Franke, Geiger: "A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells." in: The EMBO journal, Vol. 1, Issue 6, pp. 761-9, 1984 (PubMed).

Hanukoglu, Fuchs: "The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins." in: Cell, Vol. 33, Issue 3, pp. 915-24, 1983 (PubMed).

Tseng, Jarvinen, Nelson, Huang, Woodcock-Mitchell, Sun: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).

Fuchs, Coppock, Green, Cleveland: "Two distinct classes of keratin genes and their evolutionary significance." in: Cell, Vol. 27, Issue 1 Pt 2, pp. 75-84, 1982 (PubMed).