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FADD antibody (Fas (TNFRSF6)-Associated Via Death Domain) (Sepharose)

Details for Product anti-FADD Antibody No. ABIN371639, Supplier: Log in to see
Antigen
  • MORT1
  • Mort1
  • Mort1/FADD
  • TNFRSF6
Alternatives
anti-Human FADD antibody for Western Blotting
Reactivity
Human
168
70
32
7
3
3
1
Host
Mouse
170
53
Clonality (Clone)
Monoclonal ()
Conjugate
This FADD antibody is conjugated to Sepharose
9
8
7
5
5
5
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
189
110
73
19
17
13
13
7
4
4
3
2
1
1
Supplier
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Clone 1A11
Isotype IgG1
Specificity This antibody is specific for FADD.
Characteristics Synonyms: FAS-associated death domain protein, MORT1, GIG3, FAS-associating deathdomain-containing protein, Mediator of receptor induced toxicity, Growth-inhibiting gene 3protein
Alternative Name FADD (FADD Antibody Abstract)
Background Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1a-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation. (Ref.1-6)Synonyms: FAS-associated death domain protein, FAS-associating death domain-containing protein, GIG3, Growth-inhibiting gene 3 protein, MORT1, Mediator of receptor induced toxicity
Gene ID 8772
UniProt Q13158
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, TLR Signaling
Application Notes ELISA. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer 2 mLof 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Store protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Wieder, Essmann, Prokop, Schmelz, Schulze-Osthoff, Beyaert, Dörken, Daniel: "Activation of caspase-8 in drug-induced apoptosis of B-lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3." in: Blood, Vol. 97, Issue 5, pp. 1378-87, 2001

Newton, Harris, Bath, Smith, Strasser: "A dominant interfering mutant of FADD/MORT1 enhances deletion of autoreactive thymocytes and inhibits proliferation of mature T lymphocytes." in: The EMBO journal, Vol. 17, Issue 3, pp. 706-18, 1998

Muzio, Chinnaiyan, Kischkel, ORourke, Shevchenko, Ni, Scaffidi, Bretz, Zhang, Gentz, Mann, Krammer, Peter, Dixit: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996

Cleveland, Ihle: "Contenders in FasL/TNF death signaling." in: Cell, Vol. 81, Issue 4, pp. 479-82, 1995

Boldin, Varfolomeev, Pancer, Mett, Camonis, Wallach: "A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death domain." in: The Journal of biological chemistry, Vol. 270, Issue 14, pp. 7795-8, 1995