Collagen, Type I (COL1) antibody

Antigen: Collagen, Type I (COL1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Amphibian

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN372600

Additional Information

Characteristics: Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Alternative name: Collagen type I (Telopeptide)

Swiss-Prot: P02452

Immunogen: Peptide from the Human Collagen I Alpha-1 telopeptide sequence.

Cross-Reactivity: Amphibian, Fish, Human, Mammalian, Mouse (Murine), Sheep (Ovine), Bird (Avian)

Isotype: IgG  (Matching secondary antibodies)

Description: Collagen is the most abundant protein in mammals with type 1 collagen being the mostabundant of the 29 known collagens. Type 1 collagen is integral in the strengthening andstructural support of many tissues including skin, tendon, artery walls, fibrocartilage, boneand teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that arecleaved during maturation and secretion. After cleavage of the propeptides, the mostN-terminal and C-terminal remaining sequences are known as telopeptides. Mutations inthe collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (akabrittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues ofthe helical region of alpha 1 collagen have been implicated in the prevention or delayedremoval of the procoallagen N-propeptide leading to a combined osteogenesis imperfectaand Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al. , 2005).

Specificity: Specific for the ~140 kDa telopeptide portion of the Collagen I Alpha-1 polypeptide. Species Reactivity: Tested: Human, Mouse, Rat and Sheep. Expected from sequence similarity: all Mammals, Birds and Amphibians.

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemitry: 1/100. The antibody works well for Immunohistochemistry on Paraformaldehyde-Fixed Sectionswith a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90ºC in 10 mMsodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections ofFormaldehyde-Fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen Ithat has formed fibrils in the extracellular matrix. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Azide Free

Buffer: PBS without preservatives.

Storage: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only