GABRB2 antibody (Cytoplasmic Loop)

Catalog No. ABIN372637

This Rabbit Polyclonal antibody specifically detects GABRB2 in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog and Non-Human Primate.

Quick Overview for GABRB2 antibody (Cytoplasmic Loop) (ABIN372637)

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Reactivity: Human, Rat, Mouse, Dog, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRB2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRB2 Antibody

Binding Specificity: Cytoplasmic Loop

Specificity: Specific for the ~55k Beta-2 subunit of the GABAA Receptor in Western blots.

Cross-Reactivity (Details): Species reactivity (expected):Human, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Fusion protein from the cytoplasmic loop of the Beta-2 subunit of Rat GABAA Receptor.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for GABRB2

Target: GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))

Alternative Name: GABRB2

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six Alpha's, four Beta's and four Gamma's, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for Alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a Gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different Alpha-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003).Synonyms: GABA A receptor subunit beta-2, GABRB-2, Gamma-aminobutyric acid receptor subunit beta-2

Gene ID: 25451

NCBI Accession: NP_037089

UniProt: P63138

Pathways: Sensory Perception of Sound, Synaptic Membrane