This Metabotropic Glutamate Receptor 1 antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
This antibody is specific for the ~125k monomer and the ~250k mGluR1a dimer. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.
Cross-Reactivity (Details)
Species reactivity (tested):Rat.
Purification
Affinity Chromatography.
Immunogen
Peptide from the C-terminal region of Rat mGluR1a.
Western blot: 1/1000. Immunohistochemistry: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
The metabotropic Glutamate Receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004, Francesconi et al., 2004, Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003).Synonyms: Gprc1a, Metabotropic glutamate receptor 1, Mglur1