RPH3A antibody (pSer234)
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- Target See all RPH3A Antibodies
- RPH3A (Rabphilin 3A (RPH3A))
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Binding Specificity
- pSer234
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Reactivity
- Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RPH3A antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognizes the ~82k Rabphilin protein phosphorylated at Ser234. The immunolabeling of the Rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse.
Species reactivity (tested):Rat. - Purification
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- Immunogen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of Rabphilin 3A.
- Isotype
- IgG
- Top Product
- Discover our top product RPH3A Primary Antibody
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- Application Notes
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Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Target
- RPH3A (Rabphilin 3A (RPH3A))
- Alternative Name
- Rabphilin-3A / RPH3A (RPH3A Products)
- Synonyms
- 2900002P20Rik antibody, AU022689 antibody, AW108370 antibody, CG11556 antibody, Dmel\\CG11556 antibody, exophilin-1 antibody, fc61h10 antibody, rph3a antibody, wu:fc61h10 antibody, rabphilin 3A antibody, Rabphilin antibody, rabphilin-3a, putative antibody, rabphilin-3A antibody, rabphilin 3A S homeolog antibody, rabphilin 3A homolog (mouse), a antibody, RPH3A antibody, Rph3a antibody, Rph antibody, Smp_145120 antibody, Tsp_00335 antibody, rph3a.S antibody, rph3aa antibody
- Background
- Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994, Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001).Synonyms: Exophilin 1, Exophilin-1, KIAA0985, Rabphilin 3A
- Gene ID
- 171039
- NCBI Accession
- NP_598202
- UniProt
- P47709
- Pathways
- Synaptic Vesicle Exocytosis
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