RPH3A antibody (pSer234)

Catalog No. ABIN361489

Product Details anti-RPH3A Antibody

Target: RPH3A (Rabphilin 3A (RPH3A))

Binding Specificity: pSer234

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPH3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for the ~82k rabphilin protein phosphorylated at Ser234. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: mouse

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-RPH3A antibody (ABIN361489)

Stephens, Powell, Taraska, Harris: "Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).

Target Details for RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: RPH3A (RPH3A Products)

Synonyms: 2900002P20Rik antibody, AU022689 antibody, AW108370 antibody, CG11556 antibody, Dmel\\CG11556 antibody, exophilin-1 antibody, fc61h10 antibody, rph3a antibody, wu:fc61h10 antibody, rabphilin 3A antibody, Rabphilin antibody, rabphilin-3a, putative antibody, rabphilin-3A antibody, rabphilin 3A S homeolog antibody, rabphilin 3A homolog (mouse), a antibody, RPH3A antibody, Rph3a antibody, Rph antibody, Smp_145120 antibody, Tsp_00335 antibody, rph3a.S antibody, rph3aa antibody

Background: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994,Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001). Anti-Phospho-Ser234 Rabphilin 3A Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

Molecular Weight: '82 kDa

Gene ID: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis