GADD34 antibody (C-Term)

Catalog No. ABIN374070

The Goat Polyclonal anti-GADD34 antibody (ABIN374070) specifically detects GADD34 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for GADD34 antibody (C-Term) (ABIN374070)

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GADD34 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GADD34 Antibody

Binding Specificity: C-Term

Sequence: C-AAALDLSGRR G

Specificity: This antibody detects GADD34 / PPP1R15A at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein according to NP_055145.3. Genename: PPP1R15A

Application Details

Application Notes: ELISA: 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 75 kDa band observed in lysates of cell line HEPG2(calculated MW of 73.5 kDa according to NP_055145.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GADD34

Target: GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))

Alternative Name: GADD34 / PPP1R15A

Background: A protein phosphatase whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents.Synonyms: Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog, Protein phosphatase 1 regulatory subunit 15A

Gene ID: 23645

NCBI Accession: NP_055145

UniProt: O75807

Pathways: ER-Nucleus Signaling, Unfolded Protein Response