GRB2 antibody (C-Term)

Catalog No. ABIN374150

The Goat Polyclonal anti-GRB2 antibody is suitable to detect GRB2 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for GRB2 antibody (C-Term) (ABIN374150)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GRB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GRB2 Antibody

Binding Specificity: C-Term

Sequence: C-PRNYVTPVNR NV

Specificity: This antibody detects GRB2 / ASH at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide from C-Terminus of the protein sequence according to NP_002077.1, NP_987102.1 Genename: GRB2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in Jurkat lysate. Immunohistochemistry: 0.1-0.3 μg/mL. In paraffin embedded Human Tonsil, stains mantlezone and to a lessser extent germinal centre.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2 / ASH

Background: Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2

Gene ID: 2885

NCBI Accession: NP_002077

UniProt: P62993

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation