HLTF antibody (C-Term)

Catalog No. ABIN374218

Product Details anti-HLTF Antibody

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This HLTF antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-KQAKINEIRT LIDL

Specificity: This antibody detects RNF80 / HLTF at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dag, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_003062.2, NP_620636.1 Genename: HLTF

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-1 μg/mL. Approx 120 kDa and 50 kDa bands seen in Jurkat cell lysate. Please note that the weaker smaller band may represent a novel isoform of this proteinand is in agreement with previous Western blot observations (J Biol Chem 1995 Mar3,270(9): 4575-87).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for HLTF

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Alternative Name: SMARCA3 / HLTF (HLTF Products)

Synonyms: smarca3 antibody, wu:fc33f07 antibody, HLTF antibody, SMARCA3 antibody, MGC131155 antibody, HIP116 antibody, HIP116A antibody, HLTF1 antibody, RNF80 antibody, SNF2L3 antibody, ZBU1 antibody, RUSH antibody, AF010600 antibody, BC057116 antibody, P113 antibody, Smarca3 antibody, Snf2l3 antibody, helicase like transcription factor antibody, helicase-like transcription factor antibody, helicase-like transcription factor L homeolog antibody, HLTF antibody, hltf antibody, hltf.L antibody, Hltf antibody

Background: Synonyms: DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, HIP116A, Helicase-like transcription factor, RING finger protein 80, RNF80, SNF2L3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3, ZBU1

Gene ID: 6596

NCBI Accession: NP_003062

UniProt: Q14527