PNKP antibody (Polynucleotide Kinase 3'-Phosphatase) (C-Term)

Details for Product anti-PNKP Antibody No. ABIN374239, Supplier: Log in to see
Antigen
  • Tb06.28P18.320
  • 21.m03011
  • 1810009G08Rik
  • PNK
  • EIEE10
  • MCSZ
  • C21orf124
  • C21orf97
  • PKH
  • polynucleotide kinase 3'-phosphatase
  • polynucleotide kinase 3'- phosphatase
  • pyridoxal (pyridoxine, vitamin B6) kinase
  • Tc00.1047053507017.50
  • Tc00.1047053505807.190
  • Tb927.6.1580
  • BBOV_IV000690
  • CC1G_05202
  • PGTG_20262
  • PGTG_18987
  • pnkp
  • Pnkp
  • PNKP
  • PDXK
Alternatives
anti-Human PNKP antibody for ELISA
Epitope
C-Term
14
10
9
8
5
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
54
8
6
5
4
4
4
2
2
2
2
1
Host
Goat
46
7
2
Clonality
Polyclonal
Conjugate
This PNKP antibody is un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
52
32
12
9
9
2
2
1
1
Options
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Immunogen Peptide from C Terminus of the protein sequence according to NP_009185.2 Genename: PNKP
Sequence EPRLGRLYCQ FSEG
Specificity This antibody detects PNKP at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name PNKP (PNKP Antibody Abstract)
Background Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase
Gene ID 11284
NCBI Accession NP_009185
UniProt Q96T60
Pathways DNA Damage Repair, Nucleotide Phosphorylation
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: Sucessfully used to detect recombinant human PNKP (1/1000 dilution).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Background publications Jilani, Ramotar, Slack, Ong, Yang, Scherer, Lasko: "Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24176-86, 1999 (PubMed).