Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approximately 20KDa band was detected in mitotic (i. eNocodazole arrested) HeLa whole cell lysates. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates usingprotein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, withvery faint or no signal in G1 and S phase cells (fixed using paraformaldehyde, primaryconcentration 2.5 μg/mL, using FITC anti-Goat). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.Synonyms: Ubiquitin carrier protein C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C