SIRT4 antibody (C-Term)
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- Target See all SIRT4 Antibodies
- SIRT4 (Sirtuin 4 (SIRT4))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Pig
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SIRT4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Sequence
- SRCGELLPLI DPC
- Specificity
- This antibody detects SIRT4 at C-term.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human, Rat. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Immunogen
- Peptide from C-Terminus of the protein sequence according to NP_036372.1 Genename: SIRT4
- Top Product
- Discover our top product SIRT4 Primary Antibody
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- Application Notes
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Peptide ELISA: 1/32000. Western blot: Approx.38 kDa band observed in Human Kidney and U937 lysates(calculated MW of 35.8 kDa according to NP_036372.1). In transfected HEK293 transientlyexpressing SIRT4 a band of approx.38 kDa is observed. This band is not observed in thenon-transfected HEK293. Recommended Concentration: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: Skeletal Muscle shows spotty staining ofstriated fibres in longitudinal section. Recommended Concentration: 2-3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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Reduction of sulfamethoxazole hydroxylamine (SMX-HA) by the mitochondrial amidoxime reducing component (mARC)." in: Chemical research in toxicology, Vol. 27, Issue 10, pp. 1687-95, (2015) (PubMed).
: "Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase." in: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33583-92, (2007) (PubMed).
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Reduction of sulfamethoxazole hydroxylamine (SMX-HA) by the mitochondrial amidoxime reducing component (mARC)." in: Chemical research in toxicology, Vol. 27, Issue 10, pp. 1687-95, (2015) (PubMed).
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- Target
- SIRT4 (Sirtuin 4 (SIRT4))
- Alternative Name
- SIRT4 (SIRT4 Products)
- Synonyms
- SIRT4 antibody, im:7139453 antibody, SIR2L4 antibody, 4930596O17Rik antibody, sirtuin 4 antibody, SIRT4 antibody, sirt4 antibody, Sirt4 antibody
- Background
- SIRT4 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.Synonyms: NAD-Dependent ADP-Ribosyltransferase Sirtuin-4, SIR2-like 4, SIR2L4, SIRT-4, sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), sirtuin 4, sirtuin type 4, sirtuin-4
- Gene ID
- 23409
- NCBI Accession
- NP_036372
- UniProt
- Q9Y6E7
- Pathways
- Negative Regulation of Hormone Secretion
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