Paired Box 3 antibody (N-Term)

Catalog No. ABIN374426

Product Details anti-Paired Box 3 Antibody

Target: Paired Box 3 (PAX3)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Paired Box 3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: TTLAGAVPRM M-C

Specificity: This antibody detects PAX3 at N-term. This antibody is expected to recognise all 7 reported human isoforms (as represented by NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1 and NP_852126.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1. Genename: PAX3

Application Details

Application Notes: Peptide ELISA: Antibody Limit Dilution: 1/64000. Western blot: Preliminary experiments gave an approx 30-35 kDa band in Human Kidneylysates at 1 μg/mL. Immunohistochemistry: 3 μg/mL. In paraffin embedded Human Oesophagus showsnuclear staining of cells in the Squamous Epithelium.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for Paired Box 3

Target: Paired Box 3 (PAX3)

Alternative Name: PAX3 (PAX3 Products)

Synonyms: PAX3 antibody, cdhs antibody, hup2 antibody, pax-3 antibody, ws1 antibody, xpax3 antibody, Pax3 antibody, pax3 antibody, pax3b antibody, xPax3-B antibody, CDHS antibody, HUP2 antibody, WS1 antibody, WS3 antibody, Pax-3 antibody, Sp antibody, splotch antibody, zgc:92547 antibody, paired box 3 antibody, paired box 3 L homeolog antibody, paired box 3a antibody, PAX3 antibody, pax3 antibody, pax3.L antibody, Pax3 antibody, pax3a antibody

Background: This protein is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2,13)(q35,q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.Synonyms: HUP2, Paired box protein Pax-3, Pax-3

Gene ID: 5077

NCBI Accession: NP_000429

UniProt: P23760

Pathways: Sensory Perception of Sound, Tube Formation