Paired Box 3 (PAX3) (N-Term) antibody

Details for Product No. ABIN185216
Antigen
  • PAX3
  • cdhs
  • hup2
  • pax-3
  • ws1
  • xpax3
  • Pax3
  • pax3
  • pax3b
  • xPax3-B
  • CDHS
  • HUP2
  • WS1
  • WS3
  • Pax-3
  • Sp
  • splotch
  • zgc:92547
  • paired box 3
  • paired box 3 L homeolog
  • paired box 3a
  • PAX3
  • pax3
  • pax3.L
  • Pax3
  • pax3a
Epitope
N-Term
20
11
10
8
4
4
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
102
44
33
10
8
8
8
8
4
4
3
3
2
2
1
1
1
Host
Goat
76
23
7
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
4
3
3
3
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
89
54
27
11
8
6
3
3
3
2
2
1
1
1
Options
Purpose PAX3
Immunogen Peptide with sequence TTLAGAVPRMM-C, from the N Terminus of the protein sequence according to NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1.
Blocking Peptide Blocking peptide for this product available: ABIN370498
Sequence TTLAGAVPRM M
Isotype IgG
Specificity This antibody is expected to recognize all 8 reported isoforms (NP_852122.1, NP_000429.2, NP_039230.1, NP_852123.1, NP_852124.1, NP_852126.1, NP_852125.1, NP_001120838.1).
Predicted Reactivity Human, Rat, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name PAX3 (PAX3 Antibody Abstract)
Background PAX3, WS1, CDHS, HUP2, paired box gene 3 (Waardenburg syndrome 1), paired domain gene 3, paired box homeotic gene 3 (Waardenburg syndrome 1), paired box 3, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, paired box homeotic gene 3, paired domain gene HuP2
Gene ID 5077
NCBI Accession NP_852122, NP_000429, NP_039230, NP_852123, NP_852124, NP_852126, NP_852125, NP_001120838
Pathways Sensory Perception of Sound, Tube Formation
Application Notes

DS WB Results: Approx 45 kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2 kDa according to NP_852125.1 and of 44.8 kDa according to NP_852126.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Paired Box 3 (PAX3) (N-Term) antibody (ABIN185216) ABIN185216 (3µg/ml) staining of paraffin embedded Human Oesophagus. Microwaved antige...
Product cited in: Hsieh, Yao, Lai, Yang: "Transcriptional repression activity of PAX3 is modulated by competition between corepressor KAP1 and heterochromatin protein 1." in: Biochemical and biophysical research communications, Vol. 349, Issue 2, pp. 573-81, 2006 (PubMed).

Background publications Tsukamoto, Nakamura, Niikawa: "Isolation of two isoforms of the PAX3 gene transcripts and their tissue-specific alternative expression in human adult tissues." in: Human genetics, Vol. 93, Issue 3, pp. 270-4, 1994 (PubMed).

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