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DS WB Results: Approx 45 kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2 kDa according to NP_852125.1 and of 44.8 kDa according to NP_852126.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.
DS IHC Results: In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.