Peptide ELISA: 1/64000. Western Blot: 0.01-0.03 μg/mL. Approx 48 kDa band observed in Human Kidney, Liver andLung lysates. The observed molecular weight corresponds to earlier findings withantibodies from different sources. In transfected HEK293 transiently expressing Fumarasea band of approx.58 kDa is observed. This band is not observed in the non-transfectedHEK293. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
FH (Fumarate hydratse) catalyzes the conversion of fumarate to malate. It is a key enzyme in the tricarboxylic acid (TCA) cycle. It exists in a cytosolic form and an N terminal extended form. The N terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. FH also acts as a tumor suppressor.Synonyms: HLRCC, LRCC, MCL, MCUL1, mitochondrial Fumarate hydratase