PHLPP2 antibody (C-Term)

Catalog No. ABIN374772

The Goat Polyclonal anti-PHLPP2 antibody (ABIN374772) specifically detects PHLPP2 in WB. The antibody is reactive with Human samples.

Quick Overview for PHLPP2 antibody (C-Term) (ABIN374772)

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PHLPP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHLPP2 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to PHLPP2.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-PHEEDRTEPPEEFD, from the C Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PHLPP2

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Alternative Name: PHLPP2

Background: PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.Synonyms: KIAA0931, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like, PHLPPL

Gene ID: 23035

NCBI Accession: NP_055835

UniProt: Q6ZVD8

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway