FGF21 antibody (Internal Region)

Catalog No. ABIN374880

Product Details anti-FGF21 Antibody

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGF21 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CQRPDGALYG SLH

Specificity: This antibody detects FGF21.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_061986.1.

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 2000. Western blot: 1-3 μg/mL. Approx 22 kDa band observed in Human Liver lysates (calculatedMW of 22.2 kDa according to NP_061986.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-FGF21 antibody (ABIN374880)

Cyphert, Ge, Kohan, Salati, Zhang, Hillgartner: "Activation of the farnesoid X receptor induces hepatic expression and secretion of fibroblast growth factor 21." in: The Journal of biological chemistry, Vol. 287, Issue 30, pp. 25123-38, (2012) (PubMed).

Target Details for FGF21

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Alternative Name: FGF21 (FGF21 Products)

Synonyms: FGF21 antibody, fibroblast growth factor 21 antibody, FGF21 antibody, fgf21 antibody, Fgf21 antibody

Background: FGF21 is a growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors and stimulates insulin independent glucose uptake by adipocytes.Synonyms: FGF-21, Fibroblast growth factor 21

Gene ID: 26291, 9606

UniProt: Q9NSA1

Pathways: RTK Signaling