IL12B antibody (Internal Region)

Catalog No. ABIN374932

Product Details anti-IL12B Antibody

Target: IL12B (Interleukin 12 beta (IL12B))

Binding Specificity: Internal Region

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-EARQQNKYEN YS

Specificity: This antibody reacts to Il12b / Il12p40.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_032378.1.

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Aazide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: Interleukin-12 beta / IL12B (IL12B Products)

Synonyms: CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, IL-12 p40 antibody, il12b antibody, il12p40.b antibody, zgc:152789 antibody, IL-12.p40 antibody, IL12p40 antibody, IL12B antibody, CLMF p40 antibody, interleukin 12B antibody, interleukin 12b antibody, interleukin 12Ba antibody, IL12B antibody, Il12b antibody, il12ba antibody

Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2

Gene ID: 3593

NCBI Accession: NP_002178

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation