NR1I2 antibody (all Isoforms, Internal Region)

Catalog No. ABIN375099

Product Details anti-NR1I2 Antibody

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Binding Specificity: all Isoforms, Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR1I2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes PXR / NR1I2. It is expected to recognize all reported isoforms (NP_071285.1, NP_148934.1, NP_003880.3).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 1-3 μg/mL. An additional band of unknown identity was also consistentlyobserved at 35 kDa. This band was successfully blocked by incubation with the immunizingpeptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NR1I2

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Alternative Name: NR1I2 (NR1I2 Products)

Synonyms: BXR antibody, ONR1 antibody, PAR antibody, PAR1 antibody, PAR2 antibody, PARq antibody, PRR antibody, PXR antibody, SAR antibody, SXR antibody, PXR.1 antibody, PXR.2 antibody, PXR1 antibody, mPXR antibody, bxr antibody, nr1i2 antibody, onr1 antibody, xonr1 antibody, nuclear receptor subfamily 1 group I member 2 antibody, nuclear receptor subfamily 1, group I, member 2 antibody, nuclear receptor subfamily 1 group I member 2 S homeolog antibody, NR1I2 antibody, Nr1i2 antibody, nr1i2.S antibody, nr1i2 antibody

Background: The human nuclear pregnane X receptor (PXR) activates cytochrome P450-3A expression in response to a wide variety of xenobiotics and plays a critical role in mediating dangerous drug-drug interactions. The human PXR is related to the mouse Pxr1, which they had cloned and shown to be activated by dexamethasone, pregnenolone 16-alpha-carbonitrile (PCN), and other compounds known to induce expression of the CYP3A1 gene, the predominant form of CYP3A in rat liver and intestine. Northern blot analysis detected most abundant expression in liver, colon, and small intestine, transcripts of 2.6, 4.3, and 5 kb were present in each of these tissues.Synonyms: Nuclear receptor subfamily 1 group I member 2, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, PXR, Pregnane X receptor, Steroid and xenobiotic receptor

Gene ID: 8856, 9606

UniProt: O75469

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway