PIK3CA antibody (Internal Region)

Catalog No. ABIN375147

The Goat Polyclonal anti-PIK3CA antibody (ABIN375147) specifically detects PIK3CA in IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat, Cow and Dog samples.

Quick Overview for PIK3CA antibody (Internal Region) (ABIN375147)

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PIK3CA antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PIK3CA Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to PIK3CA / P110alpha.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/128000. Immunohistochemistry on paraffin sections: 2 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PIK3CA

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Alternative Name: PIK3CA

Background: Synonyms: 5-bisphosphate 3-kinase catalytic subunit alpha isoform, EC=2.7.1.153, PI3-kinase p110 subunit alpha, PI3K, PIK3CA, Phosphatidylinositol-4, PtdIns-3-kinase p110

Gene ID: 5290, 9606

UniProt: P42336

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling