GRIK1 antibody (C-Term, Internal Region)

Catalog No. ABIN375203

Product Details anti-GRIK1 Antibody

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Binding Specificity: C-Term, Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIK1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to GLuR5. This antibody is expected to recognise both reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography

Immunogen: Peptide with sequence QCKQTHPTNSTS, from the internal region (near the C Terminus) of the protein sequence

Application Details

Application Notes: ELISA: 1/8000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Alternative Name: Glutamate Receptor 5 / GLUR5 (GRIK1 Products)

Synonyms: GRIK1 antibody, grik1 antibody, fv36b03 antibody, wu:fv36b03 antibody, EAA3 antibody, EEA3 antibody, GLR5 antibody, GLUR5 antibody, GluK1 antibody, A830007B11Rik antibody, D16Ium24 antibody, D16Ium24e antibody, GluK5 antibody, Glur-5 antibody, Glur5 antibody, Glurbeta1 antibody, GluR5 antibody, glutamate ionotropic receptor kainate type subunit 1 antibody, glutamate receptor, ionotropic, kainate 1a antibody, glutamate receptor, ionotropic, kainate 1 antibody, GRIK1 antibody, grik1a antibody, Grik1 antibody

Background: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors (mGluRs). Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1 7, NMDA1 3, KAI 2) which comprise ligand gated calcium channels. Metabotropic glutamate receptors are G protein coupled receptors that contain 7 membrane spanning domains and a large extracellular N terminal region. When activated, they can activate phopholipases or adenylylcyclase, depending on the neuron. The group I receptors mGluR1 and mGluR5 activate phospholipase C. By screening a human brain cDNA library with a rat mGluR1 alpha cDNA under low stringency conditions, Minakami et al. (1993) and Minakami et al. (1994) isolated human cDNAs encoding mGluR5. Minakami et al. (1993) identified a longer isoform of mGluR5 (mGluR5B).Synonyms: EAA3, Excitatory amino acid receptor 3, GRIK1, Glutamate receptor ionotropic kainate 1

Gene ID: 2897

NCBI Accession: NP_000821

UniProt: P39086

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity