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ATG12 antibody (Autophagy Related 12) (AA 8-38)

Details for Product anti-ATG12 Antibody No. ABIN387986, Supplier: Log in to see
Antigen
  • APG12
  • APG12L
  • FBR93
  • HAPG12
  • 4931423H11Rik
  • A330058M13Rik
  • Apg12l
  • Atg12l
  • ATG12 autophagy related 12 homolog (S. cerevisiae)
  • autophagy related 12
  • similar to S. cerevisiae ATG12 (YBR217W) which nucleates preautophagosome formation as a conjugate with Atg5
  • ATG12
  • Atg12
Epitope
AA 8-38, N-Term
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality (Clone)
Polyclonal ()
Conjugate
This ATG12 antibody is un-conjugated
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Application
Immunofluorescence (IF), Western Blotting (WB)
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Options
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Immunogen This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 AA from the N-terminal region of human ATG12.
Clone RB2793
Isotype Ig
Specificity This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21~50 amino acids from the N-terminal region of human APG12L.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG12 (ATG12 Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
Synonyms: APG12, APG12L, FBR93, HAPG12, APG12 autophagy 12-like, APG12-like, Apg12 (autophagy, yeast) homolog, autophagy-related protein 12
Molecular Weight 15113 DA
Gene ID 9140
UniProt O94817
Research Area Apoptosis/Necrosis, Protein Modifications, Cell Structure, Cancer, Autophagy
Application Notes WB = 1:1000, IF = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ATG12 antibody (Autophagy Related 12) (AA 8-38) (ABIN387986) Western blot analysis of hAPG12L-T23 (ABIN387986) in MCF-7 cell line lysates (35 µg/l...
Western Blotting (WB) image for anti-ATG12 antibody (Autophagy Related 12) (AA 8-38) (ABIN387986) Western blot analysis of hAPG12L-T23 (ABIN387986) in mouse brain tissue lysates (35 µ...
Immunohistochemistry (IHC) image for anti-ATG12 antibody (Autophagy Related 12) (AA 8-38) (ABIN387986) Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L a...
Product cited in: Ma, Huang, Chen, Halliday: "Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease." in: Neuropathology and applied neurobiology, Vol. 36, Issue 4, pp. 312-9, 2010 (PubMed).

Background publications Tanida, Nishitani, Nemoto, Ueno, Kominami: "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing." in: Biochemical and biophysical research communications, Vol. 296, Issue 5, pp. 1164-70, 2002 (PubMed).

Tanida, Tanida-Miyake, Komatsu, Ueno, Kominami: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Mizushima, Sugita, Yoshimori, Ohsumi: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Ueno, Kumagai, Kijima, Kishimoto, Hosoe: "Cloning and tissue expression of cDNAs from chromosome 5q21-22 which is frequently deleted in advanced lung cancer." in: Human genetics, Vol. 102, Issue 1, pp. 63-8, 1998 (PubMed).