anti-PIAS4 antibody (Protein Inhibitor of Activated STAT, 4) (AA 48-79)

Details for Product anti-PIAS4 Antibody No. ABIN388061
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Antigen
Synonyms piasg, piasy, zmiz6, LOC100219439, PIASY, Pias-gamma, Piasg, ZMIZ6, pias4, wu:fc54c11, wu:fi18c10, wu:fi20e09, wu:fk93f11, zgc:66410
Epitope
AA 48-79, N-Term
(40), (26), (15), (14), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(85), (34), (12), (2), (1), (1), (1)
Host
Rabbit
(91), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
This PIAS4 antibody is un-conjugated
(6), (6), (6), (6), (6), (6)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(90), (58), (46), (9), (9), (3), (3), (3), (1)
Pubmed 5 references available
Quantity 400 μL
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Immunogen This PIASy antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-79 AA from the N-terminal region of human PIASy.
Clone RB0680
Isotype Ig
Specificity This PIASy antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56~86 amino acids from the N-terminal region of human PIASy1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PIASy (PIAS4 Antibody Abstract)
Background PIASy1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway, the wnt pathway and the steroid hormone signaling pathway. PIASy1 is involved in gene silencing, and promotes PARK7 sumoylation.
Synonyms: E3 SUMO-protein ligase PIAS4, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, PIAS-gamma, PIASy, PIAS4, PIASG
Molecular Weight 56504 DA
Gene ID 51588
UniProt Q8N2W9
Research Area Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Pathways JAK/ STAT Signaling
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Protein Inhibitor of Activated STAT, 4 (PIAS4) (AA 48-79), (N-Term) antibody The anti-PIASy polyclonal antibody (ABIN388061) is used in Western blot to detect PIA...
anti-Protein Inhibitor of Activated STAT, 4 (PIAS4) (AA 48-79), (N-Term) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Shima, Suzuki, Sun et al.: "Activation of the SUMO modification system is required for the accumulation of RAD51 at sites of DNA damage." in: Journal of cell science, Vol. 126, Issue Pt 22, pp. 5284-92, 2013 (PubMed).

Albor, El-Hizawi, Horn et al.: "The interaction of Piasy with Trim32, an E3-ubiquitin ligase mutated in limb-girdle muscular dystrophy type 2H, promotes Piasy degradation and regulates UVB-induced keratinocyte apoptosis through ..." in: The Journal of biological chemistry, Vol. 281, Issue 35, pp. 25850-66, 2006 (PubMed).

Background publications Imoto, Sugiyama, Muromoto et al.: "Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 34253-8, 2003 (PubMed).

Liu, Gross, ten Hoeve et al.: "A transcriptional corepressor of Stat1 with an essential LXXLL signature motif." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3203-7, 2001 (PubMed).

Liu, Liao, Rao et al.: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998 (PubMed).

Validation Images
Catalog No. ABIN388061
291.50 $
Plus shipping costs $45.00
Quantity
Price
400 μL
291.50 $

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