Bax (BH3 Domain Specific) antibody

Antigen: Bax

Epitope: BH3 Domain Specific

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388094

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Bax (BH3 Domain Specific)

Gene ID: 581

Swiss-Prot: Q07814

Immunogen: This Bax antibody was developed against a synthetic peptide between 51~81 amino acids from the N-terminal region (BH3 domain) of human Bax.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: apoptosis regulator BAX

Specificity: This antibody was developed against a synthetic peptide selected from the N-terminal region (BH3 domain) of human Bax.

Molecular Weight: 24220 DA

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Cell Structure, Cancer

Restrictions: For Research Use only

Images

Bax Antibody BH3(ABIN388094) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Bax antibody detected the Bax protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Bax Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using Bax Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.