BAX antibody (N-Term)

Catalog No. ABIN2792125

Cited in 2+ publications. The Rabbit Polyclonal anti-BAX antibody (ABIN2792125) specifically detects BAX in WB and IHC. The antibody is reactive with Human, Pig, Cow and Dog samples.

Quick Overview for BAX antibody (N-Term) (ABIN2792125)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Pig, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BAX Antibody

Binding Specificity: N-Term

Purpose: BAX Antibody - N-terminal region

Sequence: MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Pig: 100%

Characteristics: This is a rabbit polyclonal antibody against BAX. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BAX

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAX antibody (ABIN2792125)

De Martino, Marfé, Longo, Fiorito, Montagnaro, Iovane, Decaro, Pagnini: "Bid cleavage, cytochrome c release and caspase activation in canine coronavirus-induced apoptosis." in: Veterinary microbiology, Vol. 141, Issue 1-2, pp. 36-45, (2010) (PubMed).

Honda, Abe, Hiroki, Honda, Iwanuma, Yanagisawa, Ide: "Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis." in: Journal of muscle research and cell motility, Vol. 28, Issue 4-5, pp. 243-7, (2007) (PubMed).

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX

Background:

Background Information: BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.

Gene Name: BCL2-associated X protein

Alternative Symbols: BCL2L4

Protein Name: Apoptosis regulator BAX

Molecular Weight: 21kDa

Gene ID: 581

NCBI Accession: NP_620116

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response