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Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 89-122), (cleaved), (C-Term) antibody

Details for Product No. ABIN388484
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Antigen
Synonyms Map1lc3, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, 1010001C15Rik, Atg8, LC3b, Mpl3, zbs559
Epitope
AA 89-122, cleaved, C-Term
(25), (11), (9), (9), (6), (6), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(115), (44), (36), (24), (24), (14), (13), (4), (2), (1)
Host
Rabbit
(105), (14), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(9), (7), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunofluorescence (IF), Immunocytochemistry (ICC)
(63), (55), (32), (23), (23), (20), (18), (17), (11), (4), (2), (1), (1)
Pubmed 7 references available
Quantity 400 µL
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Catalog No. ABIN388484
291.50 $
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Immunogen This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 AA from human Cleaved LC3B.
Clone RB15839-RB28608
Isotype Ig
Specificity This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96~125 amino acids from the C-terminal region of human cleaved-LC3 (APG8b).
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LC3B
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 Da
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes IF = 1:10-50, ICC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.277 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 89-122), (cleaved), (C-Term) antibody Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody (ABIN388484) detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 89-122), (cleaved), (C-Term) antibody (2) SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with ABIN388484 antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
Product cited in: Jutten, Keulers, Schaaf et al.: "EGFR overexpressing cells and tumors are dependent on autophagy for growth and survival." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 2013 (PubMed).

Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

General English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

Validation Images
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