ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) (N-Term), (AA 2-31) antibody

Details for Product No. ABIN388486
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Antigen
Epitope
N-Term, AA 2-31
(12), (9), (8), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(114), (43), (41), (25), (25), (24), (24), (1), (1), (1)
Host
Rabbit
(90), (21), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(77), (47), (27), (20), (15), (14), (11), (8), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN388486
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Immunogen This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 AA from the N-terminal region of human ATG3.
Clone RB7456
Isotype Ig
Specificity This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human APG3L.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG3
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Synonyms: APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96, 2610016C12Rik, APG3 autophagy 3-like, autophagy Apg3p/Aut1p-like, autophagy-related protein 3, hApg3
Molecular Weight 35864 DA
Gene ID 64422
UniProt Q9NT62
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) (N-Term), (AA 2-31) antibody Polyclonal antibody ABIN388486 was used in Western blot to detect human APG3L in transfected 293 cells.
Background publications Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

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