Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (N-Term) antibody

Antigen: Teratocarcinoma-Derived Growth Factor 1 (TDGF1)

Synonyms: CR, CRGF, CRIPTO, Cripto-1, CR1, cripto, cb85, tdgf1, wu:fe01d04, zgc:109829, cr1, cr1a, frl1, xcr1, frl-1, TDGF1, tdgf3, cripto-3

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388791

Additional Information

Alternative name: CRIPTO (TDGF1)

Gene ID: 6997

UniProt: P13385

Immunogen: This CRIPTO (TDGF1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26~55 amino acids from the N-terminal region of human TDGF1.

Isotype: Ig

Description: Other names: Teratocarcinoma-derived growth factor 1, Epidermal growth factor-like cripto protein CR1, Cripto-1 growth factor, CRGF, CRIPTO

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CRIPTO (TDGF1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26~55 amino acids from the N-terminal region of human TDGF1.

Molecular Weight: 21168 DA

Comments:

Background: TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers. This protein could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. It is preferentially expressed in gastric and colorectal carcinomas compared to their normal counterparts.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Stem Cells, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CRIPTO (TDGF1) Antibody (N-term) (ABIN388791) in Jurkat cell line lysates (35ug/lane). TDGF1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Yan, Liu, Luo et al.: "Dual roles of Cripto as a ligand and coreceptor in the nodal signaling pathway." in: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4439-49, 2002 (PubMed).

Gray, Harrison, Vale: "Cripto forms a complex with activin and type II activin receptors and can block activin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 9, pp. 5193-8, 2003 (PubMed).

Parisi, DAndrea, Lago et al.: "Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells." in: The Journal of cell biology, Vol. 163, Issue 2, pp. 303-14, 2003 (PubMed).