Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN389018
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Antigen
Synonyms 4930406E12Rik, AU016030, AW209092, fb33h10, im:6912200, wu:fb33h10, zgc:101044
Epitope
N-Term, AA 1-30
(14), (7), (3), (3), (2), (2), (1)
Reactivity
Human, Mouse (Murine)
(39), (17), (14), (13), (12), (12), (3)
Host
Rabbit
(37), (5)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(29), (18), (10), (8), (6), (3), (3), (2), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN389018
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Immunogen This GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human GGA1.
Clone RB4932
Isotype Ig
Specificity This GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human GGA1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name GGA1
Background GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.
Synonyms: ADP-ribosylation factor binding protein GGA1, Golgi-localized, gamma ear-containing, ARF-binding protein 1, Gamma-adaptin related protein 1
Molecular Weight 70384 DA
Gene ID 26088
UniProt Q9UJY5
Research Area Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1) (N-Term), (AA 1-30) antibody The anti-GGA1 Pab (ABIN389018) is used in Western blot to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2).
anti-Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1) (N-Term), (AA 1-30) antibody (2) A, N2a cells cotransfected with APP770 �V5 and GGA1 �myc or empty vector were immunostained for APP (Alexa488. green), GGA1 (Cy5. blue), and the Golgi marker GM130 (Cy3. red). B, The same transfection was stained for the endosomal marker EEA1 (Cy3). C, To assess cell-surface localization, APP695 �GFP and GGA1 �myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27.26(39):9913-9922)
Background publications Shiba, Takatsu, Nogi et al.: "Structural basis for recognition of acidic-cluster dileucine sequence by GGA1." in: Nature, Vol. 415, Issue 6874, pp. 937-41, 2002 (PubMed).

Zhu, Zhai, He et al.: "Crystal structure of the human GGA1 GAT domain." in: Biochemistry, Vol. 42, Issue 21, pp. 6392-9, 2003 (PubMed).

Zhai, He, Liu et al.: "The interaction of the human GGA1 GAT domain with rabaptin-5 is mediated by residues on its three-helix bundle." in: Biochemistry, Vol. 42, Issue 47, pp. 13901-8, 2003 (PubMed).

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