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HSF1 antibody (Heat Shock Factor Protein 1) (AA 278-309)

Details for Product anti-HSF1 Antibody No. ABIN389069, Supplier: Log in to see
Antigen
  • AA960185
  • HSF
  • Hsf
  • hsf1
  • HSF1
  • hsf1a
  • hsf1b
  • HSF1c
  • hstf1
  • HSTF1
  • xhsf1
  • zHSF1a
  • zHSF1b
Epitope
AA 278-309, pSumoylation Site
86
31
26
25
21
20
17
17
15
15
15
14
13
13
10
10
6
6
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
386
254
135
71
68
67
62
61
4
3
2
2
1
1
1
Host
Rabbit
278
137
46
1
Clonality (Clone)
Polyclonal ()
Conjugate
This HSF1 antibody is un-conjugated
15
15
12
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11
11
8
7
7
7
7
7
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7
7
7
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
395
243
147
144
133
116
111
52
24
7
6
5
2
1
Supplier
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Immunogen This HSF1 Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-309 AA from human HSF1 Sumoylation Site.
Clone RB2562
Isotype Ig
Specificity This HSF1 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PLVRVKEEPPSPPQSPR containing a predicted sumoylation site from the central region of human HSF1.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name HSF1 (HSF1 Antibody Abstract)
Background Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.
Synonyms: Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1
Molecular Weight 57260 Da
Gene ID 3297
UniProt Q00613
Research Area Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-HSF1 antibody (Heat Shock Factor Protein 1) (AA 278-309) (ABIN389069) Western blot analysis of HSF1 polyclonal antibody (ABIN389069) in 293 cell line lysat...
Western Blotting (WB) image for anti-HSF1 antibody (Heat Shock Factor Protein 1) (AA 278-309) (ABIN389069) Western blot analysis of HSF1 (arrow) using rabbit polyclonal HSF1 Antibody (Sumo) (A...
Immunofluorescence (IF) image for anti-HSF1 antibody (Heat Shock Factor Protein 1) (AA 278-309) (ABIN389069) Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody (ABIN389069) wi...
Background publications Wang, Grammatikakis, Siganou, Calderwood: "Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1." in: Molecular and cellular biology, Vol. 23, Issue 17, pp. 6013-26, 2003 (PubMed).