Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN389543

Checkpoint Kinase 1 (CHEK1) (pSer317) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, chk1, id:ibd2720, zgc:56093, An08g10320, AO090003000441
Epitope
»Alternatives pSer317
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Dot Blot (DB), Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Catalog no. ABIN389543
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Immunogen This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Clone RB7845-RB18425
Isotype Ig
Specificity This Phospho-CHK1-S317 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CHK1
Background Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
Synonyms: Serine/threonine-protein kinase Chk1, CHEK1, CHK1,
Molecular Weight 54420 DA
Gene ID 1111
UniProt O14757
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes Recommend dilutions: DB : 1:500,WB : 1:1000,IHC : 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications Ng, Lee, Ho et al.: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004 (PubMed).

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004 (PubMed).

Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts (290), (37), (11), (5)
Reactivities (332), (127), (124), (36), (26), (25), (14), (4), (3), (3), (2), (1), (1)
Applications (247), (136), (66), (65), (50), (37), (21), (19), (6), (3), (3), (2), (1)
Conjugates (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (39), (38), (31), (26), (13), (7), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon