NFkB cRel (pSer536) antibody

Details for Product No. ABIN389601
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Antigen
Synonyms NFkB
Epitope
pSer536
(6), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(9), (2)
Host
Rabbit
(10)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(8), (2), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN389601
291.50 $
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Immunogen This NFKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NFKB.
Clone RB5212
Isotype Ig
Specificity This Phospho-NFKB-S536 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NFKB.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NFKB
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Synonyms: Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, RELA, NFKB3,
Molecular Weight 60219 DA
Gene ID 5970
UniProt Q04206
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-NFkB cRel (pSer536) antibody Western blot analysis of Phospho-NFKB-pS536(M) (ABIN389601) in mouse kidney tissue lysates (35 µg/lane). NFKB (arrow) was detected using the purified polyclonal antibody.
anti-NFkB cRel (pSer536) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Yeh, Yeh, Chuang et al.: "Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26143-8, 2004 (PubMed).

Bohuslav, Chen, Kwon et al.: "p53 induces NF-kappaB activation by an IkappaB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26115-25, 2004 (PubMed).

Jang, Yoon, Shin et al.: "PIAS3 suppresses NF-kappaB-mediated transcription by interacting with the p65/RelA subunit." in: The Journal of biological chemistry, Vol. 279, Issue 23, pp. 24873-80, 2004 (PubMed).

Jeong, Radonovich, Brady et al.: "HTLV-I Tax induces a novel interaction between p65/RelA and p53 that results in inhibition of p53 transcriptional activity." in: Blood, Vol. 104, Issue 5, pp. 1490-7, 2004 (PubMed).

Zamora, Meroño, Viñas et al.: "Recruitment of NF-kappaB into mitochondria is involved in adenine nucleotide translocase 1 (ANT1)-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38415-23, 2004 (PubMed).

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