Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pT157) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Epitope: pT157

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389616

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Phospho-p27Kip1-T157

Gene ID: 1027

Swiss-Prot: P46527

Immunogen: This Phospho-p27Kip1-T157 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T157 of human p27Kip1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1,

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T157 of human p27Kip1.

Molecular Weight: 22073 DA

Application Details

Application Notes: "The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100"

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

The anti-Phospho-p27Kip1-T157 Pab (ABIN389616) is used in Western blot to detect Phospho-p27Kip1-T157 in HL60 tissue lysate

Dot blot analysis of anti-p27Kip1-T157 Phospho-specific Pab (ABIN389616) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.