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SIRT3 antibody (Sirtuin 3) (AA 250-279)

Details for Product anti-SIRT3 Antibody No. ABIN390178, Supplier: Log in to see
Antigen
  • zgc:158774
  • sirtuin
  • SIR2L3
  • 2310003L23Rik
  • AI848213
  • Sir2l3
  • sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
  • sirtuin 3
  • sirt3
  • SIRT3
  • Sirt3
Epitope
AA 250-279, C-Term
55
14
14
11
9
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
210
72
38
13
6
4
3
3
3
3
3
2
1
1
1
1
1
Host
Rabbit
140
56
29
7
2
Clonality (Clone)
Polyclonal ()
Conjugate
This SIRT3 antibody is un-conjugated
16
16
10
10
8
8
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
214
162
95
35
11
11
5
5
5
2
2
2
1
1
Options
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Immunogen This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 AA from the C-terminal region of human SIRT3.
Clone RB01967
Isotype Ig
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SIRT3 (SIRT3 Antibody Abstract)
Background Function
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

Cellular Location
Mitochondrion matrix.

Tissue Location
Widely expressed.

Synonyms: SIR2-like protein 3, mSIR2L3, NAD-dependent deacetylase sirtuin-3,Sir2l3, Sirt3
Molecular Weight 43573 Da
Gene ID 23410
UniProt Q9NTG7
Research Area Cancer, Proteases, Cell Structure
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Immunohistochemistry (IHC) image for anti-SIRT3 antibody (Sirtuin 3) (AA 250-279) (ABIN390178) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-SIRT3 antibody (Sirtuin 3) (AA 250-279) (ABIN390178) Western blot analysis of SIRT3 antibody (ABIN390178) in Y79 cell line lysates (35 µg/...
Western Blotting (WB) image for anti-SIRT3 antibody (Sirtuin 3) (AA 250-279) (ABIN390178) Sirt3 Antibody (G265) (ABIN390178) western blot analysis in mouse kidney tissue lysat...
Product cited in: Takumida, Takumida, Katagiri, Anniko: "Localization of sirtuins (SIRT1-7) in the aged mouse inner ear." in: Acta oto-laryngologica, pp. 1-12, 2015 (PubMed).

He, Hu, Shi, Weidert, Lu, Xu, Huang, Kelley, Xie: "Activation of the aryl hydrocarbon receptor sensitizes mice to nonalcoholic steatohepatitis by deactivating mitochondrial sirtuin deacetylase Sirt3." in: Molecular and cellular biology, Vol. 33, Issue 10, pp. 2047-55, 2013 (PubMed).

Kamarajan, Alhazzazi, Danciu, Dsilva, Verdin, Kapila: "Receptor-interacting protein (RIP) and Sirtuin-3 (SIRT3) are on opposite sides of anoikis and tumorigenesis." in: Cancer, Vol. 118, Issue 23, pp. 5800-10, 2012 (PubMed).

Parker, Vazquez-Manrique, Tourette, Farina, Offner, Mukhopadhyay, Orfila, Darbois, Menet, Tissenbaum, Neri: "Integration of ?-catenin, sirtuin, and FOXO signaling protects from mutant huntingtin toxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 36, pp. 12630-40, 2012 (PubMed).

Caton, Holness, Bishop-Bailey, Sugden: "PPAR?-LXR as a novel metabolostatic signalling axis in skeletal muscle that acts to optimize substrate selection in response to nutrient status." in: The Biochemical journal, Vol. 437, Issue 3, pp. 521-30, 2011 (PubMed).

Xiong, Salazar, Patrushev, Alexander: "FoxO1 mediates an autofeedback loop regulating SIRT1 expression." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5289-99, 2011 (PubMed).

Alhazzazi, Kamarajan, Joo, Huang, Verdin, DSilva, Kapila: "Sirtuin-3 (SIRT3), a novel potential therapeutic target for oral cancer." in: Cancer, Vol. 117, Issue 8, pp. 1670-8, 2011 (PubMed).

Pillai, Sundaresan, Kim, Gupta, Rajamohan, Pillai, Samant, Ravindra, Isbatan, Gupta: "Exogenous NAD blocks cardiac hypertrophic response via activation of the SIRT3-LKB1-AMP-activated kinase pathway." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3133-44, 2010 (PubMed).

Sundaresan, Gupta, Kim, Rajamohan, Isbatan, Gupta: "Sirt3 blocks the cardiac hypertrophic response by augmenting Foxo3a-dependent antioxidant defense mechanisms in mice." in: The Journal of clinical investigation, Vol. 119, Issue 9, pp. 2758-71, 2009 (PubMed).

Sundaresan, Samant, Pillai, Rajamohan, Gupta: "SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70." in: Molecular and cellular biology, Vol. 28, Issue 20, pp. 6384-401, 2008 (PubMed).

Background publications Kawamura, Uchijima, Horike, Tonami, Nishiyama, Amano, Asano, Kurihara, Kurihara: "Sirt3 protects in vitro-fertilized mouse preimplantation embryos against oxidative stress-induced p53-mediated developmental arrest." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2817-28, 2010 (PubMed).