Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb) (N-Term) antibody

Antigen: Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb)

Synonyms: HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, GZB, CCP1, Ctla1, Ctla-1, AI553453, CCP-1/C11, RNKP-1, MGC156565, GZMB

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390495

Additional Information

Alternative name: GZMB

Gene ID: 3002

UniProt: P10144

Immunogen: This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10~39 amino acids from the N-terminal region of human GZMB.

Isotype: Ig

Description: Other names: Granzyme B, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11, GRB, CTLA1, CSPB, CGL1, GZMB

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10~39 amino acids from the N-terminal region of human GZMB.

Molecular Weight: 27688 DA

Comments:

Background: Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of GZMB antibody (N-term) (ABIN390495) in mouse stomach lysates (35ug/lane). GZMB (arrow) was detected using the purified Pab.

GZMB Antibody (N-term) (ABIN390495) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GZMB Antibody (N-term) (ABIN390495) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Gaafar, Aljurf, Al-Sulaiman et al.: "Defective gammadelta T-cell function and granzyme B gene polymorphism in a cohort of newly diagnosed breast cancer patients." in: Experimental hematology, Vol. 37, Issue 7, pp. 838-48, 2009 (PubMed).

Girnita, Webber, Brooks et al.: "Genotypic variation and phenotypic characterization of granzyme B gene polymorphisms." in: Transplantation, Vol. 87, Issue 12, pp. 1801-6, 2009 (PubMed).

Hagn, Schwesinger, Ebel et al.: "Human B cells secrete granzyme B when recognizing viral antigens in the context of the acute phase cytokine IL-21." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1838-45, 2009 (PubMed).