Valosin Containing Protein (vcp) (C-Term), (AA 726-755) antibody

Details for Product No. ABIN390907
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Antigen
Synonyms ALS14, IBMPFD, TERA, p97, 3110001E05, CDC48, p97/VCP, p93, wu:fd16d05, wu:fj63d11
Epitope
C-Term, AA 726-755
(15), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(81), (36), (34), (12), (12), (7), (3), (1), (1), (1)
Host
Rabbit
(66), (22)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(78), (31), (21), (13), (13), (10), (10), (5), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN390907
291.50 $
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Immunogen This VCP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 726-755 AA from the C-terminal region of human VCP.
Clone RB21276
Isotype Ig
Specificity This VCP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 733~763 amino acids from the C-terminal region of human VCP.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Pig (Porcine),Rat (Rattus),Xenopus laevis,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name VCP
Background VCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
Synonyms: Transitional endoplasmic reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, VCP
Molecular Weight 89322 DA
Gene ID 7415
UniProt P55072
Research Area Neurology
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Valosin Containing Protein (vcp) (C-Term), (AA 726-755) antibody Western blot analysis of VCP Antibody (C-term) (ABIN390907) in Jurkat, 293, MDA-MB231 cell line lysates (35 µg/lane). VCP (arrow) was detected using the purified polyclonal antibody.
anti-Valosin Containing Protein (vcp) (C-Term), (AA 726-755) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with VCP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Valosin Containing Protein (vcp) (C-Term), (AA 726-755) antibody (3) Flow cytometric analysis of MDA-231 cells using VCP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Validation Images
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