Activin A Receptor, Type IIB (ACVR2B) (N-Term) antibody

Antigen: Activin A Receptor, Type IIB (ACVR2B)

Synonyms: FOP, ALK2, SKR1, TSRI, ACTRI, ACVR1A, ACVRLK2, ACTRIIB, ActR-IIB, MGC116908, ActRIIB, MGC118477, actr2b, actrIIb, wu:fj97d11, ACVR2B, Acvr2b, actriib, MGC89569, actr-iib

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391162

Additional Information

Alternative name: ACVR2B

Gene ID: 93

UniProt: Q13705

Immunogen: This ACVR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ACVR2B.

Isotype: Ig

Description: Other names: Activin receptor type 2B [Precursor], Activin receptor type IIB, ACTR-IIB, ACVR2B,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACVR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ACVR2B.

Molecular Weight: 57639 DA

Comments:

Background: Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. ACVR2B (activin A type IIB receptor) displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of ACVR2B N-term S14 (ABIN391162) in Jurkat cell line lysates (35ug/lane).ACVR2B (arrow) was detected using the purified Pab.

Western blot analysis of anti-ACVR2B Antibody (N-term) (ABIN391162) in mouse heart, kidney and brain lysates (35ug/lane). ACVR2B (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Shin, Wang, Yim et al.: "Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7607-16, 2003 (PubMed).

Casagrandi, Bearfield, Geary et al.: "Inhibin, activin, follistatin, activin receptors and beta-glycan gene expression in the placental tissue of patients with pre-eclampsia." in: Molecular human reproduction, Vol. 9, Issue 4, pp. 199-203, 2003 (PubMed).

Martins da Silva, Bayne, Cambray et al.: "Expression of activin subunits and receptors in the developing human ovary: activin A promotes germ cell survival and proliferation before primordial follicle formation." in: Developmental biology, Vol. 266, Issue 2, pp. 334-45, 2004 (PubMed).

Harrison, Gray, Fischer et al.: "An activin mutant with disrupted ALK4 binding blocks signaling via type II receptors." in: The Journal of biological chemistry, Vol. 279, Issue 27, pp. 28036-44, 2004 (PubMed).