Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) (C-Term) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12)

Synonyms: DLK, MUK, ZPK, ZPKP1, MEKK12, PK, Zpk, MGC77370, zgc:77370, MGC151798, map3k12a, DKFZp459K2433

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391319

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ZPK (C-term)

Gene ID: 7786

Swiss-Prot: Q12852

Immunogen: This ZPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830~859 amino acids from the C-terminal region of human ZPK.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Mitogen-activated protein kinase kinase kinase 12, Leucine-zipper protein kinase, MAP3K12, M3KC, Mixed lineage kinase, Dual leucine zipper bearing kinase, DLK, MAPK-upstream kinase, MUK

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ZPK.

Molecular Weight: 93188 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure, Transcription Factors, Signaling, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of anti-ZPK C-term Pab (ABIN391319) in HL-60 cell lysate (lane A) and in mouse kidney tissue lysate (lane B). ZPK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.