Sphingosine Kinase 2 (SPHK2) (AA 590-620), (C-Term) antibody

Details for Product No. ABIN391349, Supplier: Log in to see
Antigen
  • CG2159
  • CG32484
  • Dmel\\CG32484
  • SK2
  • Sphk2
  • sk2
  • DKFZp469F224
  • SK 2
  • SK-2
  • SPK 2
  • SPK-2
  • C76851
  • Sphingosine kinase
  • sphk2
  • Sphingosine kinase 2
  • sphingosine kinase 2
  • sphK2
  • Sk2
  • SPHK2
  • Sphk2
Epitope
AA 590-620, C-Term
29
25
15
7
5
5
4
3
3
2
2
2
1
1
1
Reactivity
Human
153
44
43
Host
Rabbit
113
42
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
7
7
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
107
90
89
36
32
18
16
13
6
2
1
Options
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Immunogen This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 AA from the C-terminal region of human SPHK2.
Clone RB03625
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SPHK2 (SPHK2 Antibody Abstract)
Background Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Synonyms: Sphingosine kinase 2, SK 2, SPK 2
UniProt Q9NRA0
Research Area Cancer, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Cell Cycle
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Sphingosine Kinase 2 (SPHK2) (AA 590-620), (C-Term) antibody (ABIN391349) The anti-SphK2 Pab (ABIN391349) is used in Western blot (Lane 1) to detect c-myc-tagg...
Immunohistochemistry (IHC) image for anti-Sphingosine Kinase 2 (SPHK2) (AA 590-620), (C-Term) antibody (ABIN391349) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-Sphingosine Kinase 2 (SPHK2) (AA 590-620), (C-Term) antibody (ABIN391349) SPHK2 Antibody (A605) (ABIN391349) western blot analysis in HepG2 cell line lysates (...
Product cited in: Sun, Zhang, Wang, Wang, Ma, Lei, Zhou, Sun, Lu, Liu, Han: "Down-regulation of Sphk2 suppresses bladder cancer progression." in: Tumour biology, 2015 (PubMed).

Coste, Brenneis, Linke, Pierre, Maeurer, Becker, Schmidt, Gao, Geisslinger, Scholich: "Sphingosine 1-phosphate modulates spinal nociceptive processing." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32442-51, 2008 (PubMed).

Background publications Liu, Sugiura, Nava, Edsall, Kono, Poulton, Milstien, Kohama, Spiegel: "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform." in: The Journal of biological chemistry, Vol. 275, Issue 26, pp. 19513-20, 2000 (PubMed).

Shutler, Glassco, Kang, Korneluk, Mueller: "Genomic structure of the human D-site binding protein (DBP) gene." in: Genomics, Vol. 34, Issue 3, pp. 334-9, 1996 (PubMed).